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We can however look to side effects associated with other peptides to hypothesize what we might need to look out for

These outcomes are typically evaluated using migration assays, actin filament staining, and kinase phosphorylation analysis

Research applications include comparative receptor pharmacology assays examining the mechanistic differences across all three compounds, multi-receptor pathway activation studies in cell line models, and in vitro assays designed to characterise the distinct signalling profiles of each compound within a standardised experimental framework

A unit of 40 g of protein lysate was incubated with SDS sample buffer for 5 min at 90 C, resolved on a 10% polyacrylamide gel and electroblotted on a polyvinylidene difluoride membrane