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Description

Boyao, Y

Hepatocyte organoid culture For hepatocyte organoid cultures, the isolated PHHs (EpCAM-negative fraction) were mixed with Matrigel (Corning) or BME2 (AMSBIO), and 12,50050,000 cells were seeded in 50-l domes per well in 24-well plates and incubated at 37 C and 5% CO 2