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Changes to the microbial phyla and genera observed in the 5A-1MQ-treated group switched from WD to LD could have been driven by additive, synergistic, or independent mechanisms relative to those induced by diet switch alone, which could not be determined with the current study design that lacked a group treated with 5A-1MQ and maintained on WD

To confirm this, we performed immunohistochemistry on sectioned TA muscles and observed that CD3 + T cells are detected more abundantly at day 5 compared to day 0 in the TAs of young, old and geriatric mice (Fig

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