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Muller, P., Vousden, K.H.: Mutant p53 in cancer: new functions and therapeutic opportunities

It is not generally in a cells best interest to overexpress a protein

doi:10.1016/j.bbacli.2014.11.002 [22] Kashihara Y, Terao Y, Yoda K, et al

Use species-level signals (e.g., DHE-HPLC for O 2 via 2-OH-E, compartment-targeted HyPer/roGFP-Orp for H 2 O 2 , peroxiredoxin redox state as a fast sentinel) to attribute source and location, use footprints (F-isoprostanes, protein carbonyls/nitrotyrosine, 8-oxoG) to gauge exposure, add buffer indices (GSH/GSSG, TAC) and iron axis (labile iron pool, oxidized phospholipids, GPX4 context) to estimate OH/ferroptosis propensity