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Description

J.GethingP

Evaluation of plasma neurodegenerative biomarkers for diagnosing minimal hepatic encephalopathy and predicting overt hepatic encephalopathy in Chinese patients with hepatic cirrhosis

Cloning, vector construction, and transformation The expression primers for the lactate dehydrogenase gene ( ldhA , XM_002176401.1), D-lactate dehydrogenase gene ( D-LCR , XM_002179948.1), glyoxalase I gene ( Glo1 , XM_002185099.1, GlxI , XM_002180396.1), glyoxalase II gene ( Glo2 , XM_002185180.1), G protein-coupled receptor gene ( GPCR , XM_002176403.1), and pPha-T1 expression vector (AF219942.1) were designed for overexpression and RNA silence (Table S1), respectively, according to the whole-genome sequencing results of P