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Lipopolysaccharide (LPS), a structural part of the outer membrane of Gram-negative bacteria, often results in intestinal immune challenge, thereby compromising intestinal integrity, nutrient transport and utilization ( Macleaya cordata, a medicinal plant belonging to the Papaveraceae family, is traditional Chinese medicine and has been widely used as a natural feed additive in animal husbandry in China, Europe, and North America ( The gastrointestinal tract is not only the largest immunological organ but also the primary site of digestion and absorption for animals ( Lactobacillus , and decreases the abundance of Salmonella and Corynebacterium ( Therefore, the purpose of this study was conducted to investigate the effects of dietary MCE supplementation on growth performance, intestinal antioxidant function, intestinal morphology, and inflammation in broiler chickens challenged with LPS, and further to detect gut microbiota and plasma metabolites to reveal the underlying mechanism