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Recombinant protein purification For GST-tagged protein purification, pGEX-KG (GST), pGEX-KG-Ssu72 (GST-Ssu72), and pGEX-GSK3 (GST-GSK3) (Addgene, #15898) were expressed in Rosette competent cells and incubated overnight at 18 C with 0.5 mM or 0.2 mM isopropyl--D-1-thiogalactopyranoside (IPTG)

For the body to resist these damages, it possesses a range of endogenous antioxidants, primarily enzymatic antioxidants (superoxide dismutase (SOD), glutathione peroxidase (GPX), glutathione S-transferase (GST), glutathione reductase (GR), thioredoxin reductase (TrxR), catalase (CAT), etc.), and nonenzymatic antioxidants (glutathione (GSH), bilirubin, ubiquinone, etc.) [11]

No imputation was performed for the missing data

Sometimes, a dog hears a happy, high-pitched laugh or an upbeat 'praise tone.' Brain regions associated with processing rewarding sounds become more active