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Mueller, M.J

siRNA and plasmid transfection For siRNA transfection, cells were transiently transfected with target-specific siRNA oligonucleotides (GenePharma) using the JetPRIME transfection reagent (Polyplus, Bas-Rhin, France) in accordance with the manufacturers protocol, and downstream assays were performed 48 h post-transfection

Before injecting the water into the LIPO-C vial, inject an equal volume of air into the vial first to equalise pressure