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The Connectivity Map data comes from cell lines treated in vitro

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This vitamin is vital for the organisms cell reproduction, growth, tissue and protein synthesis and blood formation

After the centrifuge, cells were resuspended in 20 mL proliferation medium containing Neural Stem Cell Basal Medium (SCM003, Merck Millipore), B27 without vitamin A (12587010, Thermo Fisher Scientific, Massachusetts, USA), GlutaMAX (35050061, Thermo Fisher Scientific), antibiotics (100 IU/mL penicillin and 100 g/mL streptomycin), 1 mg/mL epidermal growth factor (E9644, Sigma-Aldrich), and 1 mg/mL fibroblast growth factor 2 (100-18B, PeproTech, NJ, USA), and incubated at 37 C with 5% CO 2