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Peptides were separated using a high-pH-compatible 250 3 4.6 mm C18 Waters BEH X-Bridge peptide separation technology (PST) 3.6 mM or Phenomenex Cell Reports 22, 27842796, March 6, 2018 2793 Kinetex Evo 2.6 mM (Torrance, CA USA) column with identical dimensions