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Cells were then transfected with 100 L of 25 and 50 nM fluorescent siRNA complexed with AuNPs-d-PLL-PEG MR 25 (500 g/mL) and AuNPs-d-PLL-PEG-FA MR 25 (500 g/mL) and incubated for four hours in 500 L serum-free RPMI

A small amount of air is surrounded by a thin film of water, in which detergent molecules stabilize the boundary

The two pools showed no significant differences in the frequency of modified alleles on day 3 of embryo development (Average of 81% and 83%, range 73-86%) ( Fig