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The cells were then rinsed three times with PBS solution, incubated with cell lysis buffer for 30 min, scraped off, and centrifuged at 12,000 rpm for 15 min at 4 C

32 (8), 15431551 (1999) A

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Later, the same group discovered that mice that lacked NFB in astrocytes had reduced Mn-induced glial activation and neuronal loss ( in vitro and in vivo , while LRRK2 deletion in microglia reduced cytokine release in vitro (Kim et al., 2019)