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Mend your fences: The epithelial barrier and its relationship with mucosal immunity in inflammatory bowel disease

NADH driven proton pumping Transmembrane proton pumping during the forward reaction was measured by following membrane potential generation using TMRE fluorescence at ex/em 549/573 nm in a PTI Quantamaster in 1.5 ml 50 mM HEPES pH 7.5, 100 mM KCl, 50 l transhydrogenase proteoliposomes, 4 U/ml glutathione reductase, 1 mM oxidized glutathione, 0.2 M TMRE and 200 M NADH

204 Symptoms of deficiency include neurological problems like neuropathy, cognitive dysfunction, and depression, as well as anemia, dermatitis, glossitis (inflamed tongue), and weakened immune function

Buffer DA treatment also increased HO-1 expression supporting previous evidence that DA alone promotes oxidative stress and increases HO-1 expression (Schmidt et al