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Furthermore, membranes were washed with PBS-T and incubated overnight at 4 C with primary antibodies for TH at dilution of 1:1000 (Thermo Fisher Scientific, #OPA1-04050) and the sites of antibodyantigen reaction were visualized with horseradish peroxidase-conjugated secondary antibodies (P448, diluted 1:3000

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Currently, there is no recommended dose of TQ for its role in preventing photoaging

Cell and tissue data may be normalized to cell number, protein, DNA, or tissue weight, while extracellular-medium measurements should account for culture volume, cell number, incubation time, and viability