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10.3389/fnut.2022.992733 7 BollimpelliV

In recent years, changes in climatic conditions have raised the intensity of plant diseases by modifying the interaction between the host, pathogen, and environment triangle

Cells were rinsed with PBS and permeabilised with methanol at 20C for 10 min, before rinsing with PBS and blocking (1% milk (SigmaAldrich) in PBS) and incubating with anti-pERK antibody (1:150 Cell Signalling Technology 4370) in blocking solution for 1 h at room temperature followed by 3x PBS rinses and incubating with Hoechst 33342 (1g/mL Molecular Probes) and anti-rabbit AlexaFluor 546 or 568 (1:1000 Molecular Probes) in blocking solution for 1 h at room temperature

Springer, Berlin pp 111147 Rennenberg H (1982) Glutathione metabolism and possible biological roles in higher-plants