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A80-229AP, Bethyl Laboratories, Inc.) in PBS/T/M was added for 1 h at room temperature, followed by wash and detection with 1 mg/mL (100 L/well) of p -Nitrophenyl Phosphate Substrate (Sigma-Aldrich) dissolved in diethanolamine buffer (pH 9.8) (Sigma-Aldrich)

Glutathione in these fractions were alkalized and derivatized with 6-aminoquinolyl-N hydroxy succinimidyl carbamate as described previously

Oxidative stress can increase as a result of illness, environmental toxins, poor diet and other lifestyle factors

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