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The membranes were blocked with 5% BSA and incubated with primary antibodies at 4 C for 12 h, followed by secondary antibodies (HRP-conjugated Goat Anti-Mouse IgG, Proteintech, SA00001-1, 1:20000) for 1 h at room temperature

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One of the downstream effects of Abcb10 loss is the activation of a nutrient stress response mediated by the transcription factor ATF4, which regulates the expression of various genes involved in the cellular stress response