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RNA Preparation and Hybridization Analysis Total RNA was extracted using the acid guanidine thiocyanate-phenol-chloroform method [20]

1.1 Epidemiological status of HT 1.1.1 Incidence and risk factors of HT HT is the most prevalent autoimmune disorder affecting the thyroid gland, characterized by the destruction of thyroid cells due to lymphocytic infiltration and the presence of autoantibodies