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EDS specialists also recognize that prolotherapys mechanism, triggering collagen growth, is logical for a collagen-deficient condition, even if the degree of benefit is hard to predict in advance

Beyond traditional viability assays, the system captured mechanistic endpoints including mitochondrial dysfunction, oxidative stress, and fibrosis-related pathways, highlighting its suitability for mechanistic studies, biomarker discovery, and safety risk assessment

Goepfert RP, Lewin JS, Barrow MP, Gunn GB, Fuller CD, Beadle BM, Garden AS, Rosenthal DI , Kies MS, Papadimitrakopoulou V, Lai S, Schwartz DL, Hutcheson KA

The general occurrence of this dehydroalanine-derived post-translational modification thus makes a strong candidate to search by proteomic-based approaches [210] its presence in inactivated enzymes and in other proteins