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Boise Biologics Regenerative Medicine 5983 W State St #C, Boise, ID 83703 208.888.3358 [email protected] Hours: Mon, Tue, Thu 95 Wed 103 Fri 1012 Proudly serving Boise, Meridian, Eagle, Nampa, Caldwell & Kuna

Refolding of synthetic monobodies The polypeptides were dissolved in solubilization buffer (6 M guanidine-HCl, 500 mM arginine-HCl, 20 mM HEPES, pH 8.5, 150 mM NaCl, 0.5 mM TCEP-HCl) at a concentration of 0.5 mg/mL and transferred into a Slide-A-Lyzer G2 dialysis cassette with a cut-off of 3.5 kDa (Thermo Scientific)