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Cell viability and proliferation assays Parental E0771, M-Wnt, and metM-Wnt lung cells, as well as GPX4- and PC-knockdown and shScramble control E0771 and metM-Wnt lung cells, were seeded at 5 10 3 cells/well in a 96-well plate in HPLM overnight, then treated with a ferroptosis inducer (erastin or RAS-selective lethal 3 (RSL3) [26, 27]) or carboplatin across a range of concentrations, antioxidants (1mM N-acetyl cysteine, 2.5 M liproxstatin-1 [28], 2.5-5 M ferrostatin-1 [26]), or a general ROS inducer H 2 O 2 (500 M)

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