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The hydroxyl group of the Tyr residue at this site forms a hydrogen bond with the thiol group of GSH, ionizing GSH to form a stable, highly active thiolate anion, which drives GST binding, peroxidase, and isomerase reactions

Figure 1A shows the results of a Western blot probed with P-gp-specific monoclonal antibody (C219) revealing the relative expression of P-gp in total cell extracts from drug sensitive Chinese hamster ovary cells (AuxB1), AuxB1 colchicine-selected drug resistant cells (CHO R C5), P-gp-knockout drug sensitive cells (AuxB1 Pgp ) and P-gp-knockout drug resistant cells (CHO R C5 PgpA1 , and CHO R C5 PgpA3 )

Cebrian A, Pharoah PD, Ahmed S, Smith PL, Luccarini C, Luben R, Redman K, Munday H, Easton DF, Dunning AM, Ponder BA

When GSH, and subsequent mGSH, is low, ATP production is impaired