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Using quantitative proteomics of chromatin-associated spliceosomes, we identify a previously uncharacterized intermediate complex B OTS964 , arrested by CDK11 inhibitor OTS964, that incorporates the nineteen-related (NTR) but not nineteen (NTC) complex

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This information is then used for evaluation of the translational diffusion coefficient ( D T ) used via the Stokes-Einstein equation for calculation of the hydrodynamic radius ( R h or R S ) of particles in a liquid (which is the effective size of a particle as it moves through a solution, including any associated solvent molecules), finding a distribution of particle sizes within a sample that allows for the calculation of the size distribution/polydispersity index (PDI), as well as providing estimates for the relative amount of mass or number of particles in a given size range