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Vascular dysfunction in diabetes is associated with activation of protein kinase C and enhanced production of glycolytic intermediates, advanced glycation end-products, and reactive oxygen species because of increased glucose metabolism [41]

therefore, total HIF-1 protein accumulated to comparable levels across groups, while DHA still enhanced HIF-1 ubiquitination

1B), the protein could not be unfolded within the measurable temperature range indicating that sensitivity of the protein against the two modes of denaturants is different