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Apoptosis assay Apoptosis was detected using the Annexin V-FITC Apoptosis Detection Kit (Beyotime) according to the manufacturers instructions

Months 3-6: Approaching the Maintenance Phase What the trials documented: By around month four (week 17), the REDEFINE protocol reached the 2.4 mg maintenance dose, and weight loss continued to accrue beyond that point through the 68-week primary endpoint (Aronne et al., 2025)