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Samples were resuspended in 0.1% trifluoroacetic acid (TFA) and desalted by reversed-phase HPLC on a Phenomenex Jupiter C4 column (250 mm 2.0 mm, 300 pore size) with a linear gradient from 10% to 95% of solvent B (0.07% TFA in 95% acetonitrile) in 30 min, at a flow rate of 200 L/min using an Agilent Technologies 1100 HPLC (Agilent Technologies, USA) 3

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This educational guide reviews what is known from molecular biology, exercise studies, preclinical models, safety literature, and regulatory databases without giving personal dosing or injection advice 1 2

Pre and postoperative photographs are shared for comparison, with personal reviews of surgeons and facilities, preoperative planning, where to source properly qualified manual lymphatic drainage therapists, postoperative complications and realistic outcomes