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This finding is also supported by several genetic variations seen in ASD patients related to the transmethylation/transsulfuration pathway, where glutathione is one of the byproducts (1618)

Western blot Cells were lysed in the buffer composed of 60 mM Tris (Bio-Shop, Canada), 2% SDS (Bio-Shop, Canada), 10% sucrose (Bio-Shop, Canada), and 1% protease inhibitor cocktail (Sigma Aldrich, USA), followed by sonication for 15 s in 8 cycles with 1 min pause between cycles and centrifugation at 12,000 g for 10 min

These findings underscore that GSH regulates ferroptosis not only through peroxide detoxification but also by coordinating iron trafficking and Fe-S cluster integrity, thereby coupling redox defense to core metabolic fitness