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Thus NO produced in the gut, for example, will not influence its actions in the central nervous system (CNS)

2.3 Western blot and antibodies The equal amounts (30 g) of protein lysed from aortic tissue and HUVECs, were separated by SDS-PAGE and then transferred onto polyvinylidene fluoride (PVDF) membranes (Merck Millipore, IPVH00010)

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Glutation tereduksi (GSH) adalah spesies antioksidan aktif yang secara langsung membersihkan spesies oksigen reaktif dan radikal bebas, sedangkan glutation teroksidasi (GSSG) terbentuk ketika dua molekul GSH dihubungkan melalui ikatan disulfida setelah oksidasi