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Cryo-EM grid preparation and data collection PilQ nozzle-enriched CsCl gradient fractions were dialyzed overnight against Tris buffer (10 mM Tris-HCl, pH 8.0) at 4 C and incubated with about one-fifth of the volume of DEAE Sepharose (Sigma) equilibrated with Tris buffer for 30 minutes

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NO promotes inflammatory cell recruitment, stimulates endothelial and fibroblast activity, and is essential for new blood vessel ingrowth and perfusion of the regenerating tissue [95]