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Following acclimation for seven days, mice were single-housed and maintained on HFD for 4 additional weeks

It inhibits NNMT (nicotinamide N-methyltransferase), an enzyme regulating metabolism, fat storage, and NAD+ consumption.[4][27][6] NNMT methylates nicotinamide, a NAD+ breakdown product, diverting it from recycling and reducing available NAD+

SIRT1 is part of a family of proteins called sirtuins, known as longevity genes. It uses NAD+ as a fuel source to repair DNA, reduce inflammation, and improve stress resistance

Always seek the advice of your physician or other qualified health provider with any questions you may have regarding a medical condition