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The cell pellets were suspended in 1 mL of DPBS, centrifuged (500 rpm, 5 min), and the supernatant was eliminated again

Several factors, such as oxidative stress, dysregulation of adipocytokines, the inflammasome, anti-inflammatory modulators like secretoglobin and IL-37, and the presence of other comorbidities inducing systemic inflammation may contribute to the development of inflammatory conditions in individuals with asthma and obesity

Squalene synthase influences various metabolites in the mevalonate (MVA) pathway, including coenzyme Q10, a lipophilic antioxidant, and its inhibition can sensitize cells to ferroptosis (Shimada et al., 2016)

However, an inconsistency still appears with the role of routine screening of concentration of tHcy and its respective treatment [80]