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The LOD and limit of quantification (LOQ) were calculated mathematically by the relationship between the standard error () of the calibration curve and its slope (S) using the multiplier according to ICH [50]ICH Q2 (R1), Validation of analytical procedures: text and methodology, in Proceedings of the International Conference on Harmonization, Geneva, Switzerland, 2005

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Results: In the NAFLD group at the baseline, most participants were men (53%), 40% had type II diabetes, the mean age was 51 years and the distribution between fibrosis stages F0/F1/F2/F3/F4 was 3%/26%/36%/23%/11%

Tumor development and volume were assessed each four days, and tumor volume was calculated as a simplified volume of an ellipsoid (length width 2 0.5)