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What is more noteworthy was that the GSH/GSSG level of B16 cells decreased rapidly and decreased to about 0 at 6 h, which may be due to the GSH-responsive transformation of NSBSO in B16 cells (will be illustrated in Fig

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a , HT1080 OE GFP-GPX4 WT and HT1080 OE GFP-GPX4 R152H were treated with 4 M RSL3, 30 g/ml cycloheximide, and 100 M a-Tocopherol for 0, 2, 4, or 6 hours before Western Blot analysis of GPX4 and GADPH