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doi: 10.1021/mp500257s 416 LoY-LLiuYTsaiJ-C

Yang et al., PKM2-dependent glycolysis promotes NLRP3 and AIM2 inflammasome activation

Bone marrow-derived macrophages Bone marrow cells were harvested in DMEM + medium [DMEM containing 10% fetal bovine serum (Thermo Fisher Scientific, Grand Island, NY), 2 mM glutamate, and 100 U/ml penicillin and 1 g/mL streptomycin] from femurs of 8 week-old C57BL/6 or cybb -/- mice