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Also, NOX2 was upregulated in embryonic fibroblasts obtained from C9orf72 knockout mice, suggesting that cellular redox conditions are dysregulated by depletion of C9orf72 (Liu Y

Medium was changed to 2.0 mM glutamine after 24 h and changed daily for 4 days for cell viability or for 3 days for mRNA extraction

however these ligands have some handicaps, such as their low binding capacity, cost-efficacy issues, some problems in scale-up and purification process