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While dsRNA activates TLR3 [233, 234] and ssRNA activates TLR7 [235], RNA oligonucleotides with phosphorothioate internucleotide bonds serve as ligands for TLR8 [236]

Senescent cells can develop in all mammals in response to disease, injury, or cancerous mutations

Then, the mixture was centrifuged at 5000 x g for 10 min at 4 C, and the upper layer of phenol was transferred to a new centrifuge tube