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This behaviour likely suggests that only a single cysteine reacts with DTNB when rChTg is incubated in a stoichiometric amount with DTNB and that this residue must display hyper-reactivity toward DTNB compared to all other cysteines (Fig

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Cell Metab 3:199210 Tong WH, Jameson GN, Huynh BH, Rouault TA (2003) Subcellular compartmentalization of human Nfu, an iron-sulfur cluster scaffold protein, and its ability to assemble a [4Fe-4S] cluster