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After thawing, cells were filtered through EASYstrainer 40 m (Greiner), washed with PBS and cultivated in RPMI-1640 (Gibco) supplemented with 10% fetal bovine serum (FBS-12A, Capricorn Scientific), 200 U/mL penicillin and 200 g/mL streptomycin (Gibco)

Because the antibody titer was not normally distributed based on Levenes test, non-parametric KruskalWallis and MannWhitney tests were used to assess the statistical differences between groups

Such residues for BLC include Met60, Ser216, Leu298, and Met349, whereas analogous residues for PVC involve Ile41, Val209, Pro291, and Leu342 and for HPII contain Ile114, Ile279, Pro356, and Leu407 [29, 35]

Abstract HIV-1 is a retrovirus that integrates into host chromatin and can remain transcriptionally quiescent in a pool of immune cells