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This subtracted library was added to a monolayer of Ishikawa cells grown in a 3.5-cm tissue culture plate and incubated at 37 C for 30 min, allowing bound phage to be internalized by endocytosis

If before the animals accumulated energy when they were awake and expended it during rest, alternating periods of storage with periods of fat burning, they now begin to build energy all the time and grow fatter

This hypertrophic shift recapitulates features of endochondral ossification and contributes to cartilage calcification, tidemark duplication, and eventual osteophyte formation at joint margins (Rim et al., 2020)