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In previous reports, single modulation of G pathway and F pathway for GSH production could achieve GSH contents of 1.51.7 % [19, 22] (Table 1)

The increased physical dimensions also simplify tissue handling and cell harvesting, enabling straightforward access to constructs for endpoint analyses, for example histology and RNA sequencing, using standard laboratory protocols

In particular, AST and ALT have been used to detect and monitor hepatocellular injury

increased 5OXP was associated with increased biosynthesis whilst OPA production indicated the depletion of the sulfur-containing amino acids essential for GSH biosynthesis.35 Whilst poor nutritional status may predispose some subjects to toxicity via a reduced ability to produce GSH for NAPQI-detoxication other metabolic factors may also come into play