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(2002) Induction of radioprotective peroxiredoxin-I by ionizing irradiation, J

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Am J Nephrol 46:7381

Dephosphorylated RNAs were then immediately radiolabelled using T4 PNK and [- 32 P] ATP in rCutSmart buffer (NEB, B6004S) supplemented with 5 mM DTT (Invitrogen, 15508013) at 37 C for 12 h, then desalted using Micro Bio-Spin P-30 columns (Bio-Rad, 7326250), Micro Bio-Spin P-6 columns or Amersham MicroSpin G-25 columns (Cytiva, 27532501) and gel-purified on a urea-polyacrylamide gel