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Figure 2 3 CAR-M engineering 3.1 CAR-M manufacturing and structural design M are traditionally framed as M1 (classically activated) and M2 (alternatively activated), though polarization exists along a functional continuum, reflecting dynamic states rather than discrete categories ( CAR-M therapy manufacturing follows core CAR-T steps but differs in cell origin, separation methods, and transduction strategies ( in vitro (commonly with FBS and GM-CSF) and CAR expression is verified (e.g., GFP by flow cytometry) ( Figure 1B

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