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All lanes: Western blot - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066) at 1/1000 dilution Lane 1: 293T (Human embryonic kidney epithelial cell) whole cell lysate at 20 g Lane 2: A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 g Lane 3: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 g Lane 4: Rat heart lysate at 20 g Lane 5: Mouse ovary lysate at 20 g Lane 6: Mouse heart lysate at 20 g Lane 7: Mouse lung lysate at 20 g Secondary All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution Predicted band size: 22 kDa Observed band size: 22 kDa false Exposure time: 20s WB Lab Western blot - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (AB125066) Blocking buffer : 5% NFDM/TBST

Using a cooling centrifuge, samples of serum were centrifuged at 1800g for 15 min at 4 C for laboratory purposes

PMID 20596078

It is likely that female-specific changes in protein expression also occur in ibuprofen treated livers and these changes were not investigated