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For homologous recombination mediated BAC deletions, ~1-kilo-base pair (kb) sized 5 and 3 arms were amplified via polymerase chain reaction (PCR) and cloned into the pGEM - T - easy vector (Promega) and in between these arms, an Ampicillin (Amp)-resistant (r) gene cassette or a Kanamycin (Kan)-r cassette put between two FRT sites was subcloned

Several of these molecules have dual functions since they damage cellular components such as proteins, DNA, and lipids but, at sub-toxic concentrations, they constitute signaling molecules both for plant development, physiology, and immunity ( In this review, we focus on ROS-, RNS- and RSS-mediated oxidations occurring on cysteine thiol groups of selected proteins, referred to as redox PTMs

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Ds lors o il nest pas crit glutathion rduit , cela signifie quil est sous une forme oxyde