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This leads to an impaired conversion of primary to secondary BAs, markedly shifting the overall BA composition

About 500 ng of desalted peptide digests was separated on a 25 cm C18 column using a 1.5-h gradient elution and analyzed in a DIA mode in the Orbitrap Exploris 480 mass spectrometer (Thermo Fisher Scientific) equipped with a FAIMS unit

Reduced claudin-5 expression leads to a greater barrier permeability, and reduced occludin expression is known to alter calcium fluxes into the brain, both reducing BBB integrity [10]

Fabrication of silk sericin-anthocyanin nanocoating for chelating and saturation-visualization detection of metal ions