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For DNA synthesis, ribonucleotides have to be reduced to deoxyribonucleotides

To evaluate the mitochondrial membrane potential by flow cytometry, the cells were therefore detached by using trypLE as described in 2.1, re-suspended the cells in complete cell culture medium (1 10 6 /ml) and treated with rotenone (0.5 M, 2 h) in an eppendorph tube

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Therefore, these research findings suggest that metformins neuroprotective properties may be beneficial in managing conditions like Alzheimers disease, Parkinsons disease, and other neurodegenerative disorders where oxidative stress and inflammation are prominent