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Perturbations in oocyte mitochondrial function can disrupt these signaling pathways, thereby indirectly impairing granulosa cell function and follicular homeostasis [51, 52]

In animal or ex vivo experimental models for mechanistic investigation of peptidemetal interactions, copper bioavailability, and metal-mediated tissue signalling processes

By enhancing intracellular hydroperoxides, especially hydrogen peroxide, decreasing reduced glutathione concentration, increasing cysteine oxidation, and activating protein kinases, oxidant stress can enhance the activity of transcription factors, such as the nuclear factor kappa B and activator protein-1, which are involved in the expression of cell surface adhesion molecules, growth factors, immunoreceptors, and cytokines

Thaw individual aliquots overnight in a refrigerator never at room temperature