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Following incubation, the reactions were resolved on a 7% native polyacrylamide gel, stained with SYBR Green EMSA nucleic acid gel stain (Molecular Probe, Invitrogen), and visualized using the iBright 750 imaging system (Invitrogen) for image documentation

This protection entails the inhibition of autophagy and oxidative stress via pathways including mTOR/p70S6k 18 and PINK1/Parkin 42

To examine the possible involvement of apoptosis in cell death induced by cystine starvation and other ferroptosis inducer treatments, the pancaspase inhibitor z-VAD-FMK was used for 1 h before ferroptosis induction

The relationship of physical activity and body weight with all-cause mortality: results from the Puerto Rico Heart Health Program