Lesson 5: Vaping: Address the growing issue of vaping, analyze its consequences, and develop refusal skills with engaging activities like Fact or Myth Vaping and Veto the Vape game
Cells were trypsinized, pelleted, and washed with ice-cold phosphate-buffered saline (PBS) followed by resuspension in radioimmunoprecipitation assay (RIPA) buffer supplemented with 1X Protein Inhibitor Cocktail and phenylmethylsulfonyl fluoride (PMSF) (Roche)
NC produced more than 900 million pounds of tobacco in 1975, which was about double second-ranked Kentuckys production at that time

Some of the common causes of hemoptysis include: Chronic cough due to smoking, constant throat irritation, and fibers such as dander and cotton Bronchiectasis (damaged airways), due to cystic fibrosis Pneumonia Tuberculosis Chronic obstructive pulmonary disease Bronchitis, either short term (acute) or long term (chronic) Lung cancer Other rare causes of hemoptysis include: Congestive heart failure because of mitral stenosis A crack or cocaine use Foreign objects lodged in the airways Inflammatory or autoimmune conditions such as: Lupus Granulomatosis with polyangiitis Microscopic polyangiitis (a condition that can damage the blood vessels) ChurgStrauss syndrome (a disorder marked by blood vessel inflammation) Goodpasture disease (a life-threatening autoimmune disorder that attacks tissues in the lungs and kidneys) Behcet disease (rare disorder leading to blood vessel inflammation throughout the body) Lung abscess Benign lung tumors Parasitic infection of the lung Pulmonary arteriovenous malformations (rare vascular anomalies of the lungs) Pulmonary embolism (blood clot in the lung) An injury like a gunshot wound or car accident Use of anticoagulants (blood thinners) Endometriosis HughesStovin syndrome (consists of deep vein thrombosis) Hereditary hemorrhagic telangiectasia (an inherited disorder that causes abnormal connections between arteries and veins) Sarcoidosis (abnormal collections of inflammatory cells that form lumps known as granulomata) In some cases, while doctors may not diagnose the exact cause of hemoptysis, the condition may go away within 6 months
