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Clonal 143B osteosarcoma cells expressing jAspSnFR3/NucRFP (hereafter referred to as sensor cells) were seeded onto multi-well plates in Dulbeccos modified Eagle medium (DMEM), acclimated for 24 h, and then imaged approximately every 3 h for a total of ~72 h in an Incucyte live-cell imaging system (Methods)

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But you should seek evaluation if palpitations are frequent, prolonged, or come with breathlessness, chest pain, dizziness or fainting, or if you can feel that your pulse is fast and clearly irregular